PRICING
Lab Testing Services
Transparent pricing. No hidden fees. Every test backed by accredited laboratory analysis.
COA Testing
One-time 7 Panel testing for a single sample submission.
What's included
- Identity Verification
- Purity Analysis
- Quantity Verification
- 7 Panel COA Report
Certified Program
Full certification with ongoing quarterly re-testing with optional public profile and 20% discount on lab tests.
What's included
- Certified Badge
- Public Profile
- Quarterly Random Testing
- 5 Monthly COA Tests Included (20% off)
Your complete guide to PeptideValidation.com lab testing
Explore the core analytical methods used to evaluate peptide purity, identity, sterility, and bacterial endotoxins, together with our testing standards, certification framework, and applied testing resources.
Four core methods used in peptide quality evaluation
Purity, identity, sterility, and endotoxin testing answer different quality questions. Each guide explains the method, the information a laboratory report should contain, and the limits of the conclusions that can be drawn.
HPLC Peptide Testing
Reverse-phase HPLC separates a peptide from synthesis impurities and quantifies purity as a percentage of total peak area. It is a primary method for purity profiling, but it does not confirm molecular identity on its own.
- Measures purity via C18 hydrophobicity separation
- Detects deletion peptides & oxidized residues
- Must be paired with LC-MS for identity
LC-MS Peptide Testing
Mass spectrometry measures the exact molecular mass of a peptide and compares it to the theoretical weight of the intended sequence and helps determine whether the detected compound is analytically consistent with the intended peptide.
- Confirms mass via multiply-charged ion deconvolution
- Detects wrong sequences, oxidation, deamidation
- Required alongside HPLC — never a substitute
Sterility Testing
Confirms a batch is free of viable bacteria, fungi, and yeast via 14-day culture in two growth media. This method evaluates viable microbial contamination in the tested sample.
- FTM targets anaerobic/aerobic bacteria
- SCDM targets fungi, yeast & aerobes
- Results are interpreted against the laboratory protocol
Endotoxin Testing (LAL)
The LAL assay detects bacterial lipopolysaccharides that may remain even when viable organisms are not detected. This is a separate quality question from purity, identity, and sterility.
- Gel-Clot, Turbidimetric & Chromogenic methods
- Acceptance criteria depend on the application
- A sterile batch can still fail endotoxin
Method comparison, at a glance
No single method answers every quality question. This table shows the primary purpose of each test and the areas it does not independently evaluate.
| Method | Confirms Purity | Confirms Identity | Detects Microbes | Detects Endotoxins | Full Guide |
|---|---|---|---|---|---|
| RP-HPLC | ✔ Primary | ✘ No | ✘ No | ✘ No | HPLC Guide → |
| LC-MS (ESI) | ◑ Partial | ✔ Primary | ✘ No | ✘ No | LC-MS Guide → |
| Sterility (USP <71>) | ✘ No | ✘ No | ✔ Primary | ✘ No | Sterility Guide → |
| Endotoxin / LAL (USP <85>) | ✘ No | ✘ No | ✘ No | ✔ Primary | Endotoxin Guide → |
Standards, certification & applied testing
Beyond the four methodology guides, these two resources cover who we are and how the science applies to a real, in-market peptide.
About PeptideValidation.com
Peptide validation means checking the claim, not repeating it. Learn how our independent testing, COA verification, and vendor certification framework works — and why a logo and a PDF aren't validation.
About & Certification StandardsRetatrutide Purity Testing
A polished COA doesn't prove a retatrutide sample contains what its label claims. See how HPLC and LC-MS are applied together to separate purity, identity, and content claims for this specific investigational peptide.
Read the Retatrutide Case StudyNew here? Read in this order
If you're evaluating a COA or building a testing program for the first time, this sequence builds the full picture step by step.
Start with the overview
See why all four tests exist and how they fit together on the Peptide Testing hub page.
Learn purity
Understand chromatograms, purity grades, and what HPLC can and can't tell you in the HPLC guide.
Confirm identity
See why mass matching is required in the LC-MS guide — HPLC alone can't catch substitutions.
Quick glossary
Key terms that come up across every guide.
Ready to request independent peptide testing?
Submit a sample for independent laboratory analysis and receive documentation based on the testing methods selected for your project.
Resource guide compiled from PeptideValidation.com's published testing standards. For educational and informational purposes only — not medical advice.